GALVESTON — Researchers have discovered the mechanism behind one of the Ebola virus’ most dangerous attributes — its ability to disarm the adaptive immune system.
University of Texas Medical Branch scientists determined that Ebola short-circuits the immune system using proteins that work together to shut down cellular signaling related to interferon.
Disruption of this activity, the researchers found, allows Ebola to prevent the full development of dendritic cells that would otherwise trigger an immune response to the virus.
“Dendritic cells typically undergo a process called ‘maturation’ when they’re infected by a virus — they change shape and present antigens on their surface that tell T-cells to attack that particular virus, thus generating an adaptive immune response,” said UTMB professor Alexander Bukreyev, senior author of a paper on the discovery now online in the Journal of Virology. “But Ebola prevents dendritic-cell maturation and produces a severe infection without an effective adaptive immune response. We found that its ability to do this depends on several specific regions of two different proteins.”
Bukreyev’s research group made the discovery after a series of procedures that started with a clone of the Ebola Zaire virus strain.
Working under maximum-containment conditions in a biosafety level 4 facility in UTMB’s Galveston National Laboratory, the team introduced mutations into the virus’ genetic code at four locations thought to generate proteins that affected immune response.
Ebola’s ability to evade the human immune response is one of the factors that accounts for its high mortality rate — up to 90 percent in humans — and the notoriety that it gained after its first appearance in Zaire in 1976, in an outbreak that killed 280 people. Zaire — now the Democratic Republic of the Congo — is the home country of Ndongala Lubaki, lead author on the paper and a postdoctoral fellow at UTMB.
Other authors of the Journal of Virology paper include postdoctoral fellow Phillipp Ilinykh, assistant research lab director Collette Pietzsch, research scientist Bersabeh Tigabu, assistant professor Alexander Freiberg and Richard Koup of the National Institute of Allergy and Infectious Diseases Vaccine Research Center.
This research was supported by the John Sealy Memorial Endowment Fund and the James W. McLaughlin Endowment.